Composite

Part:BBa_K4156120

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


AND gate-HlyE

AND gate-HlyE consists of an AND logic gate ( BBa_K4156115 ) and a downstream HlyE ( BBa_K4156084 ) sequence to test the performance of the AND gate by detecting the red fluorescent signal.


Usage and Biology

The construction of this part is similar to AND gate-mRFP ( BBa_K4156124 ). The difference is that we replaced the mRFP protein with the HlyE therapeutic protein. This is to verify the therapeutic power of the recombinant strain under ANDgate control. After co-incubation of the recombinant strain with cancer cells, cell viability was monitored using the CCK8 assay to assess the therapeutic power of the recombinant strain and the reinforcing effect of AND gate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 303
    Illegal BsaI.rc site found at 464


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Categories
Parameters
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